Molecular Diagnostic Assay Kit

“Molgen Genetics Haemostatic Disease (B-Fibrinogen/FXIII) Kit” is an assay kit that utilizes real-time PCR to detect single nucleotide polymorphisms in the FGB and F13A1 genes of the blood coagulation system, as well as the detection of melting curves.input:
output:Opening Statementinput:
output:The “Molgen Genetics Haemostatic Disease (B-Fibrinogen/FXIII) Kit” is designed to detect the single nucleotide polymorphism G (-455)A in the FGB gene (encodes fibrinogen and coagulation factor I) and G103T in the F13A1 gene (encodes coagulation factor XIII A subunit) in clinical specimens (whole blood, buccal epithelium) using the real-time polymerase chain reaction (PCR) with melting curve analysis.input:
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The complex evaluation of thrombophilia risk (susceptibility to hereditary thrombophilia) considers the results of genotyping.input:
output:The “Molgen Universal Extraction Kit” can be employed to extract DNA from clinical specimens.input:
output:The Adaltis AmpliLab is recommended for use with the block-type PCR cycler CFX96 (Bio-Rad, USA).input:
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The kit includes the necessary reagents for 48 tests, as well as control samples.input:
output:Methodology Principleinput:
output:The method’s principle is predicated on the amplification of the selected human DNA region, followed by the formation of hybrid duplexes of PCR product with the specific DNA probe and the detection of duplexes melting curves.input:
output:The amplification step is comprised of repeated cycles, including primer annealing to specific sites of the DNA template, primer extension with Taq polymerase, and temperature denaturation of the DNA template.