Solution Reagent

2X DirectGO PreMix-CE is a premixed, ready-to-use solution that includes Hot Start Taq DNA polymerase, PCR enhancers, glycerol, loading dye, and reaction buffer in a 2-fold concentration.input:
output:In comparison to conventional Taq polymerase, DirectGO Taq prevents non-specific amplification as a result of mispriming and/or the formation of primer dimers before thermal cycling. Additionally, it has a 2X faster elongation rate than conventional Taq. When amplification reactions are heated at 94-95°C for ten minutes, the polymerase activity is reactivated during the initial denaturation step.input:
output:Furthermore, 2X DirectGO PreMix-CE is appropriate for direct PCR amplification from whole blood, as well as samples from cell lines or tissues. You have the option of skipping any previous DNA purification steps and directly loading a small quantity of raw material into the PCR reaction, running it in a PCR machine, and then loading it into an agarose gel for electrophoresis. The 2X DirectGO PreMix-CE is a potent instrument for DNA analysis due to its rapidity and specificity.