Research Test Kit

Our most versatile ELISpot format, designed to seamlessly integrate into your distinctive strategy. Combine it with the substrate, plate, and/or antigen of your choice.Components

Capture mAb - MT52B34
Detection mAb - biotin, MT11B37
Streptavidin-HRP enzyme conjugate

Purpose
This ELISpot Flex kit is designed to count the number of cells that secrete sheep IL-5 using the ELISpot assay. For research purposes only. Not intended for diagnostic procedures.Specifications of the item

ELISpot Application
IL-5 is the analyte.
Sheep Reactivity
Specificity - This kit is constructed from a matched pair of mAbs that facilitate the precise detection of sheep IL-5.
Cross-reactivity - The mAbs react with IL-5 from sheep and bovine IL-5. Cross-reactivity guide: For additional details, please refer to the section below.

PerformanceSheep PBMC were incubated for 40 hours in the presence (100,000 cells/well) or absence (200,000 cells/well) of PMA/ionomycin, and the number of cells secreting IL-5 was analyzed using ELISpot.

The tables below provide a summary of the species reactivities of the veterinary assays and the monoclonal antibodies that were included.

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Cross-reactivity

Confirmed by Mabtech and/or other sources. Tests conducted using ELISpot and/or ELISA.IL-5

Description of the analyte

T and NK cells are the primary producers of interleukin 5 (IL-5), a pleiotropic cytokine. The survival, differentiation, and chemotaxis of eosinophils are specifically regulated by IL-5. However, IL-5 was initially distinguished by its capacity to facilitate the growth and terminal differentiation of B cells.
Interleukin-5, IL-5, IL5, BCGF-2, and TRF are alternative names.
cell type: T cell, Th2, NK cell