Protein Sequencer

The PPSQ is an instrument that integrates a high-performance liquid chromatograph (HPLC) with an Edman reaction section to ascertain the amino acid sequences of proteins and peptides. There are two types: the PPSQ-51A, which is equipped with a single reactor, and the PPSQ-53A, which is equipped with three reactors. The amino acid sequences of multiple samples can be continuously analyzed on the PPSQ-53A in a sequential manner. In the Edman reaction section, amino acids are derivatized and cleaved in order from the N-terminal of a protein through the repeated application of Edman degradation. This leads to the production of stable PTH-amino acids. The PTH-amino acids are injected into the HPLC online, and the analysis is conducted. The chromatograms are processed using data processing software after the HPLC data is saved on the PC. The amino acid sequence estimation software is then employed to identify the amino acids and estimate the sequences.Advantages of employing Edman degradation for amino acid sequencinginput:
output:(1) Proteins with an N-terminal sequence that is guaranteedinput:
output:(2) No sample pre-treatment is necessary.(3) Differentiation of isobaric amino acids (leucine and isoleucine)Adherence to 21 CFR Part 11 of the Food and Drug Administrationinput:
output:LabSolutions PPSQ software ensures adherence to the security, user management, and audit trail requirements outlined in FDA 21 CFR Part 11 and is in accordance with FDA 21 CFR Part 11 guidelines.input:
output:1. Protectioninput:
output:Each user is identified by a username and password, and users are managed according to groups. Unique groups can be established by incorporating individuals with varying levels of access privileges.