Gastrointestinal Infection Test Kit

For in vitro diagnostic purposes. RIDA®GENE EAEC is a multiplex real-time PCR that is designed for the direct, qualitative detection of enteroaggregative E. coli (EAEC) in human stool samples. The RIDA®GENE EAEC multiplex real-time PCR is designed to assist in the diagnosis of gastroenteritis caused by enteroaggregative E. coli.input:
output:General information:A member of the Enterobacteriaceae family, Escherichia coli (E. coli) are gram-negative, facultatively anaerobic rod bacteria that move through peritrichal flagellation. E. coli are typically nonpathogenic and are a component of the normal intestinal flora of humans and numerous farm animals. Certain virulence factors (e.g., genes for toxins) are acquired by certain E. coli strains, which render them pathogenic to humans.input:
output:The virulence factors of the six known intestinal pathogenic E. coli: enterohämorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), and diffusely adherent E. coli (DAEC) can be used to differentiate them.



In 1987, enteroaggregative E. coli (EAEC) were initially identified and described in the stool of a child from Chile. The characteristic aggregative adherence (AA) phenotype of EAEC is its defining characteristic. The epithelial cell surface is adhered to by EAEC in a "stacked-brick" formation in the gold standard HEp-2 cell adherence assay. EAEC are E. coli that adhere to HEp-2 cells in an AA pattern and do not secrete heat-labile or heat-stable enterotoxins. A high molecular weight plasmid (pAA) associated with AA is present in specific EAEC strains. This plasmid contains a number of virulence genes, including aggR, aggA, aafA, agg3, and aatA.