Enzyme Reagent

The EpiDirect® MGMT Methylation qPCR Assay is a novel and innovative method that enables the direct quantification of MGMT promoter methylation through qPCR without the need for prior DNA conversion.input:
output:Our proprietary INA® chemistry is the foundation of the EpiDirect® technology, which incorporates an EpiPrimer™ oligonucleotide that specifically binds and primes the amplification of methylated target DNA.input:
output:
The assay can quantify MGMT promoter methylation in just two hours using standard real-time PCR instruments*, with as little as 0.5 ng of unconverted input DNA.input:
output:Procedure for conventional qPCR instruments

There is no requirement for DNA conversion beforehand.

Results in less than two hours
LoD methylation ranges from 1.5 to 3%.Hands-on time is minimal, with an input DNA concentration of 0.5-50 ng.

CE IVDinput:
output:Product Description: PCR master mix and oligonucleotide mix for the quantification of MGMT promoter methylation, including a positive and negative control.

Structure Prepared for Use

Reactions: forty-eightinput:
output:The EpiDirect® MGMT Methylation qPCR Assay is a semi-automated real-time Polymerase Chain Reaction (PCR) assay that is designed for the direct, semi-quantitative analysis of the methylation status of the human DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) gene promoter.input:
output:The assay is designed for the analysis of purified genomic DNA (gDNA) samples that can be used with real-time PCR systems to detect and quantify the methylation status of the MGMT promoter without the need for additional pre-treatment. Samples can be prepared using automated platforms or manual workflows and consist of Formalin-Fixed Paraffin-Embedded (FFPE) and fresh frozen biopsies.