Enzyme Reagent

Mycoplasma detection through direct culture is a time-consuming process, and certain mycoplasma species are resistant to cultivation. The presence of contaminant mycoplasma can be easily detected by verifying the bands of amplified DNA fragments in electrophoresis, allowing for the acquisition of results within a few hours with mycoplasma PCR testing. Probes labeled with radioisotopes require no preparation, nor is it necessary to determine enzyme, dNTP, or buffer concentrations. Rather, a PCR mix that is optimized and ready to use is provided.

A precipitant is included in the reaction mix of the mycoplasma detection kit to facilitate the direct loading of PCR products onto an agarose gel.

Numerous Spices for the Detection of Mycoplasma

The primer set enables the detection of a variety of mycoplasma species, including M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. bovis, M. pneumoniae, M. pirum, and M. capricolum, as well as Acholeplasma and Spiroplasma species, with enhanced sensitivity and specificity.input:
output:Avant-garde The following advantages are associated with the Mycoplasma PCR Reagent set, which is predicated on a straightforward assay protocol:input:
output:A high degree of sensitivity

Mycoplasma-specific primers with a broad range

Convenient and user-friendly: includes a full reaction mix with Taq polymerase. Necessitates no more than 10-20 minutes of actual work.sample preparation is effortless.The results are readily ascertained through a single PCR procedure.Rapid: results obtained in no more than five hoursThere is no requirement for internal control application steps.The agarose gel requires no loading dye.