Buffer Solution Reagent

A weak alkaline aqueous solution of inorganic acid and surfactant is referred to as Reagent B. The pH value falls within the range of 8.00 to 9.00. Reagent B is utilized to neutralize weak acidic solutions, which is particularly critical for DNA quantification.input:
output:It is employed in conjunction with Reagent A100 to enhance the binding efficiency of DAPI, prevent DNA degradation, and elevate the pH value of the sample.



By increasing the pH, Reagent B enables the precise quantification of DNA content in acidic cell samples.



The neutralization of acidic cell samples is crucial for the stabilization of DNA and also enhances the fluorescence signals of a variety of dyes, including Propidium Iodide (PI). For more detailed instructions, please consult the specified Application Notes.input:
output:The solution is intended for research use only (RUO) and is not intended for diagnostic or therapeutic purposes. For additional information, please consult the documents listed below. Purchase the item from our online store.input:
output:Reagent B is employed for the following:NucleoCounter® NC-100™ total cell count.

Assay for the Count of Aggregated Cells A100 and B using the NucleoCounter® NC-200™, NC-250™, and NC-3000™.